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Proliferative effects of flavan-3-ols and propelargonidins from rhizomes of Drynaria fortunei on MCF-7 and osteoblastic cells.
Chang EJ, Lee WJ, Cho SH, Choi SW
Department of Food Science and Nutrition, Catholic University of Daegu, Hayang, Gyeongbuk 712-702, Korea.
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Arch Pharm Res 2003 Aug; 26(8): 620-30.
The proliferative effects of thirty Oriental medicinal herbs on MCF-7 (estrogen-sensitive breast cancer cell line) and ROS 17/2.8 osteoblast-like cells were determined using the MTT assay. Methanol extracts from several herbs was found to show proliferative activity on the above two cell lines in the range of 5 to 100 microg/mL. Among these active herbs, the methanol extract from the rhizomes of Drynaria fortunei showed the most potent proliferative activity, and the cell proliferations were significantly increase by 136 and 158% in the MCF-7 and ROS 17/2.8 cells, respectively, when treated with 100 microg/mL. Through a bioassay-guided separation, eight flavonoids, including four new flavan-3-ols and two propelargonidins, together with the known (-)-epiafzelechin and naringin, were isolated. Their chemical structures were characterized as (-)-epiafzelechin (1), (-)-epiafzelechin-3-O-beta-D-allopyranoside (2), (-)-epiafzelechin-3-O-(6"-O-acetyl)-beta-D-allopyranoside (3), 4beta-carboxymethyl-(-)-epiafzelechin methyl ester (4), 4beta-carboxymethyl-(-)-epiafzelechin sodium salt (5), naringin (6), (-)-epiafzelechin-(4beta-->8)-4beta-carboxymethylepiafzelechin methyl ester (7) and (-)epiafzelechin-(4beta-->8, 2beta-->O-->7)-epiafzelechin-(4beta-->8)-epiafzelechin (8) by extensive 1D and 2D NMR spectroscopy. Most of these flavonoids, in the range of 10(-15) to approximately 10(-6) M, accelerated the proliferation of MCF-7 cell, with compounds 7 and 8, in the range of 10(-15) to approximately 10(-12) M, showing especially potent proliferation effects. Meanwhile, seven flavonoids, with the exception of compound 4, stimulated the proliferation of ROS 17/2.8 cells in the range of 10(-15) to approximately 10(-6) M, with compounds 5-8 especially accelerating the proliferation, in dose-dependent manners (10(-15) to approximately 10(-9) M), and their proliferative effect was much stronger than that of E2 and genistein. These results suggest that propelargonidin dimers and trimers isolated from the rhizomes of Drynaria fortunei may be useful as potential phytoestrogens, which play important physiological roles in the prevention of postmenopausal osteoporosis.
| Major Subject Heading(s) | Minor Subject Heading(s) |
>Flavonoids [pharmacology]
>Isoflavones [pharmacology]
>Plant Preparations [pharmacology]
>Polypodiaceae [chemistry]
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>Animals
>Breast Neoplasms [pathology]
>Cell Division [drug effects]
>Drugs, Chinese Herbal [chemistry]
>Flavonoids [isolation & purification]
>Humans
>Isoflavones [isolation & purification]
>Magnetic Resonance Spectroscopy
>Molecular Structure
>Osteoblastoma [pathology]
>Osteoporosis, Postmenopausal [prevention & control]
>Phytoestrogens
>Plant Preparations [isolation & purification]
>Polypodiaceae
>Rats
>Rhizome [chemistry]
>Tumor Cells, Cultured
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12967197
Publication Type: Journal Article
Citation Subset: IM
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Last Modified: 12/25/2009 |
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